5-Methyltryptophan resistant cells of Catharanthus roseus.
نویسندگان
چکیده
Several cell lines resistant to 5-methyltryptophan were selected from wild type cells of different Catharanthus roseus suspension cultures. The resistant cells had up to 30 times the normal levels of free tryptophan. Despite the increased pool size of tryptophan anthranilate synthetase activity of resistant cells was as sensitive to inhibition by L-tryptophan as wild type cells. The overproduction of tryptophan did not lead to intensified accumulation of tryptamine nor of indole alkaloids. This was supported by a low conversion of tryptophan to tryptamine in vivo and in vitro. The overproduction of one of the primary precursors was evidently not sufficient to stimulate the rate of indole alkaloid synthesis in Catharanthus cells.
منابع مشابه
Selection of Cell Lines of Catharanthus roseus with Increased Tryptophan Decarboxylase Activity
Catharanthus roseus, Cell Suspension C ultures, T ryp tophan A nalogues, R esistant Cell Lines, Tryptophan Decarboxylase, Indole A lkaloids Cell lines o f Catharanthus roseus were selected for resistance to 4 -m ethyltryptophan (4-M T) in order to get strains with increased activities o f tryp tophan decarboxylase (TD C ). This enzym e may exert a decisive regulatory control in indole alkalo id...
متن کاملMitotic Block of Human Blood Cells by Vinca herbacea, Catharanthus roseus and Colchicine Alkaloids
Catharanthus roseus (L.) G.Don is a plant which produces anticancer and anti-mitotic indole alkaloids. Colchicine is an anti-mitotic drug. Anti-mitotic effects of Vinca herbacea Waldst. & Kit. indole alkaloids is unknown. The study were evaluated the antimitotic effect of alkaloids of V. herbacea, Catharanthus roseus andcolchicineon mitosis and microtubule arrangement of human blood cells. In t...
متن کاملBiochemical and Cellular Response of Catharanthus roseus Callus Cells to Cadmium Toxicity
Catharanthus roseus is a medicinal and ornamental plant with growing attention toward its economical value. Cell suspension from C. roseus were treated with 0 to 60mM of cadmium nitrate for 1, 3 and 6 days, then cell viability was determined using trypan blue and MTT assay. Cell morphology was investigated using 0, 10, 30 and 50 mM of cadmium nitrate as selected dose for 3 days. Callus of C.ros...
متن کاملAdenine Phosphoribosyltransferase Activity in Mitochondria o f Catharanthus roseus Cells
Catharanthus roseus (= Vinca rosea), Adenine Phosphori bosyltransferase, Subcellular D istribution, Mitochondria, Purine Salvage Subcellular localization of adenine phosphoribosyltrans ferase was examined in Catharanthus roseus cells. At least 13% of the activity was observed in the particulate fraction with the rest observed in the cytosol. Both differential and Percoll density gradient cent...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Zeitschrift fur Naturforschung. Section C, Biosciences
دوره 34 7-8 شماره
صفحات -
تاریخ انتشار 1979